We developed a NanoString multiplexed gene panel (Additional file 1: Table S5) and simultaneously measured the mRNA expressions of 8 prognostic genes (BAALC, CDKN1B, ERG, MECOM/EVI1, FLT3, ID1, MN1 and WT1) as well as IL2RA in an intermediate-risk AML cohort 66 patients. The gene discussed is WT1; the disease is acute myeloid leukemia.