The IL2RA mRNA and its protein CD25 levels were assayed in 54 primary AML BM samples by TaqMan RQ-PCR and FCM respectively, in which the mRNA level was shown as 2−∆Ct of IL2RA gene relative to ABL1 gene and the expression level of CD25 protein was illustrated as percentage in leukemia blast cells. Here, ABL1 is linked to acute myeloid leukemia.