The prognosis role of PAI-1was evaluated in different subtypes of breast cancer using the Kaplan-Meier plotter (http://kmplot.com/analysis/), the results indicated that high expression of PAI-1 was correlated with poor prognosis of breast cancer, especially in HER2 negative cancers, ER negative cancers and triple negative cancers (Fig. 7a). The gene discussed is SERPINE1; the disease is breast cancer.