BCR and neoplasm: Pathological staging of the tumor (pT: pooled pT3/4 vs pT2), ISUP histological grade group of the tumor, presence of lymph nodes metastases (pN1 vs pN0) and status of the resection margins (R1 vs R0) were included into multivariate Cox regression models, together with the expression of single genes which showed statistically significant association with BCR-free survival in univariate Cox regression analysis (3571 genes with dichotomization using median of expression and 9273 genes with dichotomization using the optimized cut-off).