NLRP3 and acute monocytic leukemia: To further confirm whether NLRP3 was indispensable for inflammasome activation induced by S. suis, an nlrp3-deficient human acute monocytic leukemia THP-1 cell line (THP-1-nlrp3-/-) and a control cell line (THP-1-nlrp3+/+) were constructed using clustered regularly interspaced short palindromic repeats (CRISPR) technology.