In contrast, the addition of solubilized cholesterol at the given concentration did not significantly inhibit the IL-1β secretion induced by the NLRP3 agonist ouabain (Fig 5F and 5G). These studies indicated that inflammasome activation in response to S. suis epidemic strain SC-19 infection required the binding of SLY to host cells. Here, NLRP3 is linked to infection.