Next, we assessed the size and cell composition of IAs by counting cells of 6 different sub-types (all tumor cells, HLA+ tumor cells, CD3+, CD4+ FOXP3+, CD8+PD1+, CD20+ cells) and calculated counts of each of these subpopulations relative to the number of total tumor cells in IAs (Fig 4). The gene discussed is FOXP3; the disease is neoplasm.