Robust, dose- and time-dependent 2HG depletion has been observed across a host of cell types including human chondrosarcoma cells and mouse-model xenografts, primary human AML myeloblasts, and mutIDH1R132H glioma xenografts (IC50 range 5–13 nM for various IDH1 mutants in vitro) (68, 88, 89). The gene discussed is IDH1; the disease is central nervous system cancer.