Robust, dose- and time-dependent 2HG depletion has been observed across a host of cell types including human chondrosarcoma cells and mouse-model xenografts, primary human AML myeloblasts, and mutIDH1R132H glioma xenografts (IC50 range 5–13 nM for various IDH1 mutants in vitro) (68, 88, 89). This evidence concerns the gene IDH1 and chondrosarcoma.