CAMKK2 and breast neoplasm: Specifically, E0771 breast tumor cells were engrafted into the mammary fat pad of a [Tg(Camkk2-EGFP)C57BL/6 J] Camkk2-reporter mouse model25,26, and the immune cell repertoire was analyzed by flow cytometry using a gating strategy (outlined in Supplementary Fig. 1B) that enables resolution of tumor-associated myeloid cell subsets27.