Furthermore, in the following cancer cell lines, including esophageal cancer cell lines (EC109, EC9706 and KYSE-450), liver cancer cell lines (SMMC-7721, HepG2 and ZIP177) and gastric cancer cell lines (MGC-803, HGC-27, SGC-7901 and MKN45), we found that MTH1 was highly expressed to 1.48-, 1.73-, 1.60-, 2.35-, 1.90-, 1.73-, 2.01-, 2.07-, 1.61-, 1.14-folds, compared with those in their corresponding normal cell lines(Het-1A, L02 and GES-1), respectively (Fig. 1d–i), the same as their mRNA expression levels shown in Fig. 1j. This evidence concerns the gene NUDT1 and liver cancer.