In conclusion, our study reported the following new findings: (1) miR-3650 expression is significantly reduced in HCC tissues and cells; (2) low expression of miR-3650 is positively associated with poor survival of HCC patients and could be used as an independent prognostic predictor; (3) miR-3650 functions as a tumor suppressor in the migration and EMT abilities of HCC; (4) NFASC is a directly target mRNA of miR-3650; (5) miR-3650 inhibits HCC cells migration and EMT by binding and inhibiting NFASC. Here, NFASC is linked to hepatocellular carcinoma.