According to the final tumor weight, compared with the control group, about 73, 86, and 100% of 4T1 tumor reduction was achieved by the dosage of 5, 25, and 50 μg of TPP@NPs with irradiation, and yet irradiation or 5 μg of TPP@NPs showed negligible anti-tumor effect. This evidence concerns the gene TNS3 and neoplasm.