Since the minor allele frequencies (MAFs) provide resemblance not only between healthy control (HC) and natural clearance (NC) but also between CHB without HCC and CHB-related HCC; hence, we divided all the patients into two groups: control (NC + HC) and case (CHB without HCC + CHB-related HCC) to explore the relationship between STAT3, STAT4 polymorphisms and chronic HBV infection susceptibility. This evidence concerns the gene STAT4 and hepatocellular carcinoma.