We have investigated the effect of high expression OCT4 on the stemness characteristics of ALL MSCs, and it was clearly demonstrated that OCT4 overexpression improved proliferative capacity, which was similar to the results reported in the previous literatures [2, 15] Meanwhile, the OCT4 overexpression cells presented ESC-like characteristics expressing higher levels of stemness-related markers such as NANOG, SOX2, SSEA-4, and TRA-1-60. Here, POU5F1 is linked to acute lymphoblastic leukemia.