To demonstrate the function of HOXA5 short RNA on tumor development in vivo, male athymic nude mice (n = 5) were subcutaneously injected with HCT116 cells stably expressing HOXA5 short RNA (pEB-HOXA5 short) and mock-transfected HCT116 (pEB-mock) cells and the growing tumor masses were measured. This evidence concerns the gene HOXA5 and neoplasm.