CYP3A4 and hepatocellular carcinoma: Enhancement of CYP3A4 mRNA expression observed here (Figure 3) was comparable to that in previous reports, for example, where a dynamic perfusion culture that could supply oxygen at higher level also enhanced CYP3A4 mRNA expression in the FLC-5 hepatocellular carcinoma cell line [30] and in growth-arrested Hep G2/C3A spheroids [31].