BRAF and cancer: Of a total of over 2200 alterations in BRAF described from the 159 cancer genomic studies represented in cBioPortal, V600E mutations clearly represent the vast majority, with mutations at position K601 included within the top five next most commonly mutated codons (together with D594, N581, G466 and G469, ranging from 28–67 mutations reported, compared to > 1000 for V600, data shown in S2 Fig.