To ensure that the observed reduction in CD69 and CD86 expression was not due to effects of CK-666 on the APCs, we used Arp3 siRNA to deplete the Arp2/3 complex in B16F1 murine melanoma cells expressing the single chain anti-Igκ Ag (Figure 9—figure supplement 3A,B). This evidence concerns the gene CD69 and melanoma.