To further investigate the mechanism of the inhibitory effect of triptolide in vivo, the tumor tissues were analyzed by a TUNEL assay, immunohistochemistry with antibodies against Ki67, cleaved caspase-3 and p-JNK, and western blotting with primary antibodies against cleaved caspase-3, LC3, p-JNK, p-Akt and p-mTOR. Here, MKI67 is linked to neoplasm.