To determine if these dietary constituents were inducing transcriptional changes in the LECs we performed qRT-PCR on the hLECs and evaluated the transcript abundance of IL13 (Figure 5D) because the IL13 pathway was upregulated in LECs from people with end stage NASH (Figure 5A) and IL13 has been shown to cause defects in lymphatic branching and inhibit PROX1 expression (35). This evidence concerns the gene IL13 and metabolic dysfunction-associated steatohepatitis.