Figure 4A shows photomicrographs of microglia double labeled with DAPI (blue) and for Bcl-2 (green). The majority of microglia were found to constitutively express Bcl-2 under control conditions (Figure 4Ai), and this protein was detected both following ischemia (Figure 4Aii) and when cells were incubated in afobazole (Figure 4Aiii). Ischemia reduced the number of microglia expressing Bcl-2 by 15 ± 3% relative to control (Figure 4B). This evidence concerns the gene BCL2 and ischemia.