CASP3 and ischemia: Figure 3A shows photomicrographs of microglia double labeled with DAPI (blue) and for caspase-3 (green). There was a notable increase in the number of microglia expressing caspase-3 following ischemia (Figure 3Ai,ii) and this upregulation was reduced by afobazole treatment (Figure 3Aiii,iv). Quantification of the images showed that afobazole alone significantly decreased caspase-3 expressing cells by 20 ± 3% relative to the control and diminished the increases in caspase-3 evoked by ischemia by 56 ± 5% (Figure 3B).