To understand the role of iron in the development of PAH in these mice, we compared the iron status of PASMCs from fpnC326Yfl/fl SMMHC-CreERT2+ mice and PASMCs from fpnC326Yfl/fl controls, by measuring levels of elemental cellular iron, cellular ferritin, and expression of IRP-regulated genes. The gene discussed is WNT2; the disease is pulmonary arterial hypertension.