In univariate analysis, our result indicated that male patients, larger tumor size, advanced pT stage, advanced pN stage, advanced TNM stage, patients who did not receive postoperative chemotherapy and elevated CA19–9, CEA, Cyfra21–1 and SCC-Ag were significantly related to poor OS (p < 0.05, Fig. 1a-e, Table 2). This evidence concerns the gene CEACAM5 and neoplasm.