Here, we apply and improve on previous methods to establish human POI-iPSC lines using 4 inhibitor culture systems from 7 POI patients with a family history, including Fragile X syndrome, abnormal karyotype (45, X; 45, X/46, XX; 45, XO and 47, XXX), and the gene mutation (FIGLA and GDF9). Here, GDF9 is linked to fragile X syndrome.