CD274 and gastric cancer: PD‐L1 mRNA expression level has been measured in solid tumors, including gastric cancer and non‐small‐cell lung cancer by using RNA‐seq or ISH with RNAscope, and it was found to be highly consistent with their IHC detection results.19, 20, 32, 33 Benefiting from the unique “double‐Z” probes, independent of antibody, and visible results on tissue sections, the RNAscope method seemed to be more sensitive than IHC and more objective than RNA‐seq.