Apart from Th1 polarization, our data revealed that tumor Sdc-1 silencing significantly up-regulated the proportions of the Treg (Foxp3+CD4+) subset among CD4+ T cells of non-IBC patients when stimulated with the secretome of Sdc-1-silenced SUM-149 cells and in co-culture, as well as enhanced the proportion of the Treg (Foxp3+CD4+) subset among CD4+ T cells of normal subjects when co-cultured with Sdc-1-silenced SUM-149 cells, as compared to negative control. The gene discussed is SDC1; the disease is neoplasm.