Further analysis of the expression status of 35 genes demonstrated that FTO knockdown induced the expression of COL8A1, TEAD2, and CMTM4, which were upregulated in ICC (Table 2) and that FTO knockdown inhibited the expression of HAO2, NR5A2, CCL19, TCF21, APOA2, NTRK2, DPT FGA, and SCML4, which were downregulated in ICC (Table 2). The gene discussed is SCML4; the disease is intrahepatic cholangiocarcinoma.