In mild to moderate asthmatic children combination of immunotherapy house dust mite with N. sativa seed (15 mg/kg/day, 14 weeks) or oil (0.1 ml/kg/day, 2 weeks) improved asthma control test and asthma symptoms, while immunological changes such as decrease CD4+CD25+foxp3+Treg, increase CD4+IL-10+, and Th17 cells were not affected. This evidence concerns the gene CD4 and asthma.