We hypothesized that, if ELS disrupts stress responsivity trans-generationally via altered hippocampal Nr3c1, Nr3c2, or Bdnf expression, then adult rats with a history of ELS, as well as their offspring, will display atypical methylation and expression in our genes of interest, blunted corticosterone following a mild acute stressor, and increased anxiety-like behaviors. This evidence concerns the gene BDNF and Anxiety.