Figure 4C shows the results of immunofluorescence analyses (IFA) of intracellular parasites, under bradyzoite culture conditions, incubated with either GalNAz-αCPE4 or GalNAz-αCPE. VVA stains the cyst wall and the GalNAz-αCPE4 partially colocalizes with additional intracellular staining. On the other hand, GalNAz-αCPE only stains the cyst wall, although in low intensity. Azidosugar-treated parasites were also costained with an antibody specific to the known O-GalNAc modified cyst wall protein CST1. This evidence concerns the gene CST1 and cyst.