Our flow cytometry results showed that conditioned media of HIG2-silenced HCC cells stimulated the expression of the activated receptor NKp30, NKG2D, and CD16 on NK cells, and upregulated the expression of the effector molecules GZMB, Perforin, TNF-α, and IFN-γ. The gene discussed is TNF; the disease is hepatocellular carcinoma.