On the other hand, an inhibition of TRPV1-induced Ca2+ influxes by 3-T1AM-based TRPM8 activation through a negative feedback was observed in human corneal epithelial cells [62], and cancer cells, such as TRPM8 transfected U2osB2 osteosarcoma [63], WERI-Rb1 retinoblastoma, and human uveal melanoma (UM 92-1) [64]. The gene discussed is TRPM8; the disease is osteosarcoma.