This included some metastatic stem driver mutations (case 290, BUB1B absent in two FFPE blocks; case 308, ESR1 and PTEN absent in the two FFPE blocks; DET52, ATM not detected in ductal carcinoma in situ (DCIS) or metastatic axillary lymph nodes) and most metastatic clade driver mutations (SI2 in https://doi.org/10.17632/6cv77bry6m.1). This evidence concerns the gene ESR1 and ductal breast carcinoma in situ.