Cui et al Confirmed miRNA‐204 as a central regulator of vascular smooth cell calcification in vitro and in vivo by targeting Runx2.44 MiRNA‐141 was identified to inhibit the osteogenic differentiation of porcine VICs through a BMP‐dependent pathway.40 In contrast to previous studies, our study firstly used miRNA microarray assay to explore the real miRNAs involved in CAVD pathogenesis. Here, RUNX2 is linked to congenital bilateral aplasia of vas deferens from CFTR mutation.