Interestingly, we showed that AG1478 at a concentration of 5 μM is able to strongly inhibit the phosphorylation of EGFR and reduced the activation of down-stream pathways in chondrosarcoma cells cultured in 3D, indicating that chondrosarcoma did not show strong resistance to the drug in the 3D culture model used. The gene discussed is EGFR; the disease is chondrosarcoma.