Treatment of HEMC-SS chondrosarcoma cells cultured in 3D with AG1478 at 1 μM and 5 μM inhibits the phosphorylation of EGFR and reduced the activation of both ERK1/2 and AKT signaling pathways, however the inhibition is more pronounced when 5 μM of AG1478 were used (Figure 3E). The gene discussed is AKT1; the disease is chondrosarcoma.