Twelve genes in this pathway were significantly differentially expressed, among which Per1 (period circadian clock 1), c-Fos (FBJ osteosarcoma oncogene), Calm1 (calmodulin 1), and Gng5 (G protein subunit gamma 5) were upregulated; the remaining eight genes, including Grin1 (glutamate receptor ionotropic), Cacna1g (voltage-dependent calcium channel T type alpha-1G), Kir3.1 (potassium inwardly rectifying channel subfamily J member 3), Adcy4 (adenylate cyclase 4), Gucy1a2 (guanylate cyclase soluble subunit alpha), and so on were downregulated (Figure 5). Here, ADCY4 is linked to osteosarcoma.