Thus, immature DCs pulsed with ignored GAD65 antigen determinant GAD6578−97 (45), conditioning with IL-10 (43, 52), IL-25 (92), fungal extracts (93), Lactobacillus casei (67), carbon monoxide (94), or anti-CTLA4 Ab (95) were reported to prevent diabetes in animal models whereas DCs treated with PEGylated TLR7 ligand reduced diabetes and insulitis (38). Here, CTLA4 is linked to diabetes mellitus.