TRPM8 and Familial prostate cancer: To this end, we used calcium imaging to measure TRPM8 activation in prostate cancer cells (PC3) that overexpressed the channel after the cells were stimulated with the three agonists at concentrations used in previous studies3,4,25,26: a high dose corresponding to 200 μM of menthol, 10 μM of icilin and 100 nM of WS12 as well as two lower concentrations, including 1/10 (medium dose) and 1/100 (low dose) of the most effective drug (Fig. 1).