mRNAs of hepatocyte/hepatoma markers, such as albumin, ApoA1, CYP3A4, and AFP, were detectable in KH and Huh-7.5 cells, but not in A549 cells, indicating that KH cells, similar to Huh-7.5 cells, are derived from hepatocyte/hepatoma cells. This evidence concerns the gene AFP and hepatocellular carcinoma.