BCR and melanoma: Females had significantly higher BCR diversity measured by both Gini-Simpson (p = 0.01 by the Mann-Whitney U test) and Shannon entropy (p = 0.02) than males, and correspondingly a lower top clone proportion (p = 0.01), but no significant difference in BCR abundance or evenness (p = 0.11, p = 0.74, respectively Additional file 1: Figure S4), suggesting that female melanoma patients may have a more diverse B cell response.