Tumor-conditioned media (TCM) only, cytokine only (IL-4, IL-10, and M-CSF), TCM with IL-4 and IL-10, TCM with IL-4 and M-CSF, TCM with IL-10 and M-CSF, and TCM with IL-4, IL-10, and M-CSF (the last condition being the in vitro TAM generation method). The gene discussed is IL4; the disease is neoplasm.