We examined BTF3 expression in the immortalized non-tumorigenic prostate epithelial cell line (RWPE) and a series of PCa cell lines (LNCaP, DU145, 22RV1, VCaP and PC3) (Additional file 1: Figure S1A) and performed gain- and loss-of-function analyses. Here, BTF3 is linked to posterior cortical atrophy.