CD40LG and leptospirosis: The presumptive diagnosis of icteric leptospirosis was strengthened by detection of antileptospiral IgM antibodies with the use of ELISA in the acute phase serum specimen (38 U/ml, reference range: < 15 U/ml, SERION ELISA classic Leptospira IgG/IgM, Virion Serion, Würzburg, Germany) and confirmed by an accredited PCR method using proprietary primers at the Institute for Medical Microbiology and Hygiene at the University of Regensburg, Germany.