infection with L. amazonensis does not modify the blood levels of TNF-α (Additional file 2: Figure S2A) and IL-1β (Additional file 2: Figure S2B) during the experimental protocol in comparison with non-infected animals, suggesting that the systemic levels of these hyperalgesic cytokines have no decisive role in the development of neuroinflammation in the spinal cord in the present model. This evidence concerns the gene TNF and infection.