Specifically, in “Pathways in cancer” and “Colorectal cancer pathway” with the minimal p values, we discovered the same module of “PI3K-AKT signaling pathway”, “MAPK signaling pathway” and “NF-κB signaling pathway” in these two pathways (Additional file 1: Figures S1-S5), which could provide a novel research direction on colon cancer treatments. The gene discussed is NFKB1; the disease is malignant colon neoplasm.