In order to develop a model of insulin resistance induced by PA in hepatic cells, HepG2 cells were first exposed to rising doses of PA (0, 25, 50, 100, 150 and 200 μM) for 12, 24 or 36 h followed by 100 nM insulin stimulation for 10 min and cell viability were evaluated. This evidence concerns the gene INS and Insulin resistance.