TGFB1 and hepatocellular carcinoma: By example, TGF-β induced the expression of the gene for Limb Bud and Heart Development (LBH), an essential gene that promotes stemness and inhibits differentiation, in MDA-MB-231 but not in HCC-1954, and this lack of induction was due to DNA methylation of the LBH promoter region in HCC-1954.