Meanwhile, pathway enrichment analysis for down regulated genes, genes such as MYL5 [93], CAV2 [94], BMP1 [95], COL5A1 [96], SERPINH1 [97], COL7A1 [98], COL8A1 [99], COL13A1 [100], BGN [101], SEMA4B [102], ECM1 [103], TGFB3 [104], ANGPTL2 [105], MUC1 [106], and CTSO [107] were responsible for the invasion of many cancer cells such as cervical cancer, triple-negative breast cancer, lung cancer, esophageal squamous cell carcinoma, urothelial carcinoma, gastric cancer and breast cancer, but these genes may be liable for the invasion of GBM cells. The gene discussed is COL5A1; the disease is esophageal squamous cell carcinoma.