To investigate the actinomycin V effects on Epithelial-mesenchymal transition inhibition in human breast cancer cells, we preformed the Western blot analysis to evaluate the expression levels of the epithelial marker E-cadherin, and mesenchymal markers N-cadherin and Vimentin in MDA-MB-231 cells (Figure 6 and Figure 7). The gene discussed is VIM; the disease is breast cancer.