In addition, CAR T cells, TCR T cells, and TETARs showed a significantly higher killing of gp100-pulsed A375M melanoma cells (expected TCR and CAR response) at a 60:1 and 20:1 ratio in comparison to mock-electroporated cells, while cytotoxicity of TETARs was in general lower when compared to TCR T cells (Figure 4 and Table S3). This evidence concerns the gene PMEL and melanoma.