Then we found that low expression of circ-0001649 in the tumor tissues was associated with grade of differentiation and tumor satellite, but not with other clinicopathological features including gender, age, tumor diameter, serum α-fetoprotein (AFP), liver function (Child-Pugh stage), hepatocirrhosis, HBV infection or HCV infection (Supplementary Table 1). This evidence concerns the gene AFP and neoplasm.