Post-challenge sera from all vaccinated groups reacted against all samples (OvES, rOv-TSP-2, rOv-TSP-3, dEVs and Ov-EVs), indicating that the native extracts (OvES and EVs) as well as recombinant TSP-2 and TSP-3 are immunogenic in the course of a natural infection (S2B Fig). The gene discussed is THBS3; the disease is infection.